| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213162 |
| Chromosome: | chromosome 2 |
| Location: | 1989456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088300 | CPK6 | (1 of 1) PTHR10584:SF179 - PROTEIN MAK32; putative carbohydrate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGACGATGCGACGGGTACGCGGCTGCC |
| Internal bar code: | ATTCGCATGACGTTGTTTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 99.6435 |
| LEAP-Seq n confirming: | 1677 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTAGTGTCCACACGCT |
| Suggested primer 2: | GTGAAAGATGCAAAGACGCA |