| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213162 |
| Chromosome: | chromosome 2 |
| Location: | 1989463 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088300 | CPK6 | (1 of 1) PTHR10584:SF179 - PROTEIN MAK32; putative carbohydrate kinase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAGCGCTCCGGCATGTCGGGCCCGAC |
| Internal bar code: | TATGGGTGTAGGTAGGGCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 99.6426 |
| LEAP-Seq n confirming: | 9201 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTAGTGTCCACACGCT |
| Suggested primer 2: | GTGAAAGATGCAAAGACGCA |