Insertion junction: LMJ.RY0402.213168_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.213168
Chromosome:chromosome 11
Location:3003323
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478456 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTTGGTCTTAGGGTGGTGTCCGCGCCC
Internal bar code:ATGATATGAAGTTACTGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:855
LEAP-Seq percent confirming:59.6226
LEAP-Seq n confirming:1580
LEAP-Seq n nonconfirming:1070
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCTTCTTTGTGACGAGC
Suggested primer 2:TAACTGCTGCTGTCAGTGCC