| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213182 |
| Chromosome: | chromosome 6 |
| Location: | 6470405 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292900 | (1 of 10) PTHR12270:SF6 - GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTGCGCTTCGACCCCGGCTCCAGCGGC |
| Internal bar code: | CGTGACGGTCATTGAATAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 99.6789 |
| LEAP-Seq n confirming: | 32281 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCGTTTGGTTTCGTTTG |
| Suggested primer 2: | TATCGACGTGGACCTCATCA |