| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213208 |
| Chromosome: | chromosome 3 |
| Location: | 6562078 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195850 | MSRB4 | Methionine sulphoxide reductase; (1 of 2) PTHR10173:SF18 - METHIONINE-R-SULFOXIDE REDUCTASE B3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGAAAAAGCGTACTTGTTCACGTGTAG |
| Internal bar code: | AACCATGGGGTCGTCGATAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.6523 |
| LEAP-Seq n confirming: | 2293 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGGTCAATCACAGTCAC |
| Suggested primer 2: | GCACCGCCTCAAGTAATCAT |