| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213215 |
| Chromosome: | chromosome 3 |
| Location: | 2059494 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAAGGGCCAAGAGGGGGGCAGCGGGGGG |
| Internal bar code: | ACAAGGTGAAATAAGCGTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.0968 |
| LEAP-Seq n confirming: | 1975 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCACCCACTGCTGGTAG |
| Suggested primer 2: | AGTCCGGGAGGCAGATTTAT |