| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213217 |
| Chromosome: | chromosome 3 |
| Location: | 5246049 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183150 | (1 of 1) 3.4.24.21 - Astacin / Crayfish small-molecule proteinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACGGGGTTTGCGCCGAGGCCACATAA |
| Internal bar code: | CGCAAGTGTGCAATCCGGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.3465 |
| LEAP-Seq n confirming: | 4409 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | GCAAGCAGTTCCTCCACTTC |