| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213218 |
| Chromosome: | chromosome 2 |
| Location: | 2182193 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089608 | (1 of 1) K11136 - regulator of telomere elongation helicase 1 [EC:3.6.4.12] (RTEL1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGCCTCTCGCCAGCTTGTCTTGGGGCC |
| Internal bar code: | ACCGTGCCCCGAGATCGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 99.5822 |
| LEAP-Seq n confirming: | 2860 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTCAACACCCAACCGTC |
| Suggested primer 2: | CGTCTGGGCACCTAAACAGT |