| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213221 |
| Chromosome: | chromosome 17 |
| Location: | 5838072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739650 | MAE1,MFT1 | MATE efflux family protein; (1 of 5) PTHR11206//PTHR11206:SF94 - MULTIDRUG RESISTANCE PROTEIN // DNA-DAMAGE-INDUCIBLE PROTEIN F | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGGGACCGGCAAGACCGCCTGAGGCA |
| Internal bar code: | GAGTTCAATGGAAGTGCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 99.7506 |
| LEAP-Seq n confirming: | 2400 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCCTGCAAACTCAAGTA |
| Suggested primer 2: | TGATTATGGACGCGATGAAA |