Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.213225 |
Chromosome: | chromosome_14 |
Location: | 910468 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g613600 | HSP70H | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGGTGGCCGGTGGTCGTGCCGTTTCAATG |
Internal bar code: | GTGGGAGAACTTATCACGGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 5 |
LEAP-Seq percent confirming: | 97.6293 |
LEAP-Seq n confirming: | 453 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGAACAGTGGCAGCAGTA |
Suggested primer 2: | GTACGTCCCATGCAGCCTAT |