| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213227 |
| Chromosome: | chromosome 6 |
| Location: | 6038149 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289400 | PDE11 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCACGCACACGCCGCGACTATGGACAT |
| Internal bar code: | TCCTTCGGCGTTGTTGAAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 99.6003 |
| LEAP-Seq n confirming: | 1495 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGAGCACGCATACACAG |
| Suggested primer 2: | GAAAGGAGGGGAAGGATGAG |