Insertion junction: LMJ.RY0402.213235_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.213235
Chromosome:chromosome 13
Location:3742012
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGCTTGGCAACCTGGAGGCGCACACC
Internal bar code:CAGCAAGCTCGGGGTCCCGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:45
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:ACAAACGAAATTGTGAGCCC