Insertion junction: LMJ.RY0402.213235_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213235
Chromosome:chromosome 13
Location:3742015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTCAAAAACGGACGCACCCGCCTCGTG
Internal bar code:CGTCGATGCGTGCGCTCGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1010
LEAP-Seq percent confirming:99.7481
LEAP-Seq n confirming:7523
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:ACAAACGAAATTGTGAGCCC