| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213254 |
| Chromosome: | chromosome 9 |
| Location: | 35232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386137 | (1 of 1) PF06824 - Protein of unknown function (DUF1237) (DUF1237) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGGACAGGTGCGCCGGCGCGGAACGT |
| Internal bar code: | CGCGCCCGTTCCCAGGTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 94.4306 |
| LEAP-Seq n confirming: | 11716 |
| LEAP-Seq n nonconfirming: | 691 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCACTTGCCGTGAACTTG |
| Suggested primer 2: | AGGAATCTCAGGAATGCCCT |