| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213255 |
| Chromosome: | chromosome 7 |
| Location: | 1267702 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321700 | ADH3 | Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF281 - ALCOHOL DEHYDROGENASE RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACAGCAAGCGTGTGCACTACGCCTTG |
| Internal bar code: | AGATCATGAAGATTTGCTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.6462 |
| LEAP-Seq n confirming: | 845 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCAAGGCTTAAGTAGCG |
| Suggested primer 2: | CATTTTGTTACATGGCACGC |