| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213272 |
| Chromosome: | chromosome 14 |
| Location: | 942434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613850 | (1 of 1) K12585 - exosome complex exonuclease DIS3/RRP44 (DIS3, RRP44) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAGGGTTGGTGGGAGGTGGGGGATTG |
| Internal bar code: | TCATATGGCGATAGGGCCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.6136 |
| LEAP-Seq n confirming: | 10055 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGTAGGTAGCGGGAGG |
| Suggested primer 2: | CTCATCTAGCCTACCAGCCG |