| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213276 |
| Chromosome: | chromosome_12 |
| Location: | 3387963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497000 | FAP14 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCGTCCCACACCCGCCCGCCACCACACCA |
| Internal bar code: | ATTGAAGGCTTCGAGAGATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1115 |
| LEAP-Seq percent confirming: | 99.481 |
| LEAP-Seq n confirming: | 6901 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGCTTGATGGCGTATG |
| Suggested primer 2: | GCTATGGTCATGGCACACAC |