| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213290 |
| Chromosome: | chromosome 11 |
| Location: | 1773768 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467350 | ACO2 | Acyl-CoA oxidase/dehydrogenase; (1 of 1) PF01756//PF02770 - Acyl-CoA oxidase (ACOX) // Acyl-CoA dehydrogenase, middle domain (Acyl-CoA_dh_M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGACGTGCAGTGCACTGGCGGGAGGTGC |
| Internal bar code: | GAGCCACTCGGTGCCGGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.8856 |
| LEAP-Seq n confirming: | 1746 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTTCTTCCCACCAAAAC |
| Suggested primer 2: | TTCCTCATCAACTGCTGTGC |