| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213295 |
| Chromosome: | chromosome 11 |
| Location: | 3603937 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482001 | FAP208 | Ankyrin Repeat Flagellar Associated Protein 208; (1 of 1) K13118 - protein DGCR14 (DGCR14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGCCCCGAACCCCGCGCATGCTCGGCG |
| Internal bar code: | CCGCGGCTCGCCGCGTCAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 98.099 |
| LEAP-Seq n confirming: | 8205 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACGCACAC |
| Suggested primer 2: | CAACATGCATTACCCAGCAC |