| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213311 |
| Chromosome: | chromosome 16 |
| Location: | 5776890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678450 | Glycosyl transferase; (1 of 2) PTHR10859//PTHR10859:SF50 - GLYCOSYL TRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTGATGCTGACTCTGCGACTCTGGGC |
| Internal bar code: | ATTCGGTGATTATTACGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 98.9899 |
| LEAP-Seq n confirming: | 5292 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGCATCTTCGCCTACA |
| Suggested primer 2: | GCGTCGAAGATACTGAAGGC |