| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213344 |
| Chromosome: | chromosome 17 |
| Location: | 6750164 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744497 | (1 of 1) PF09783 - Vacuolar import and degradation protein (Vac_ImportDeg) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCCGGCCGGTTCCCACTGACGTGCCGT |
| Internal bar code: | TGTTCGCCATGCCCCAGCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.6082 |
| LEAP-Seq n confirming: | 2288 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCTGTCTTTGGAACAT |
| Suggested primer 2: | GATCCCAGCCAACTACCAGA |