| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213344 |
| Chromosome: | chromosome 17 |
| Location: | 6750211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744497 | (1 of 1) PF09783 - Vacuolar import and degradation protein (Vac_ImportDeg) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTCCTCGTCCTGCTCTGAGATGGTGGC |
| Internal bar code: | GGTGTTGGATGTAAAAGGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.4869 |
| LEAP-Seq n confirming: | 2133 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCTGTCTTTGGAACAT |
| Suggested primer 2: | GATCCCAGCCAACTACCAGA |