| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213395 |
| Chromosome: | chromosome 16 |
| Location: | 6519970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672750 | (1 of 2) K14944 - RNA-binding protein Nova (NOVA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCGGTCGCTGATTTTGATGGTGACCCGC |
| Internal bar code: | GACAATCGTTCTCGGATGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 99.7768 |
| LEAP-Seq n confirming: | 1788 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTGTCTTACAGGGCCAG |
| Suggested primer 2: | TAGCTGCTGCCCTTGTACCT |