Insertion junction: LMJ.RY0402.213415_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.213415
Chromosome:chromosome 1
Location:4509525
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g030700 PTK14 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTTTGCTGGTCATATCCACAATATTCG
Internal bar code:GGTATCCGGAATCGGGCGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:248
LEAP-Seq percent confirming:98.5677
LEAP-Seq n confirming:757
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACCCAGCTATAACCCCC
Suggested primer 2:GGTGATTGCGTCATCATTTG