| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213466 |
| Chromosome: | chromosome 1 |
| Location: | 7981284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g068742 | (1 of 4) PF01424 - R3H domain (R3H) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGCCGTGGATGACACTCGCGGTTTTG |
| Internal bar code: | CGTGACTATCTCTGTGTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 97.9403 |
| LEAP-Seq n confirming: | 2758 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTACAGCTTCTGTCAGCC |
| Suggested primer 2: | TTCTTCCGATTATTCACGCC |