| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213494 |
| Chromosome: | chromosome 4 |
| Location: | 622589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGTGATGAACAGCAGTGAACAGGTTG |
| Internal bar code: | GGGCCGGATCGATCACGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 99.7551 |
| LEAP-Seq n confirming: | 6518 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGTCCTCACCAAGGCAT |
| Suggested primer 2: | CTATAGTGCAGGGCTGAGGC |