| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213525 |
| Chromosome: | chromosome 14 |
| Location: | 270006 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609500 | PWR5 | PWR motif protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGTCGCCACTCGCGACTTCTGCGAGCC |
| Internal bar code: | CAGTTAATACATGGGGCATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 94.1202 |
| LEAP-Seq n confirming: | 6627 |
| LEAP-Seq n nonconfirming: | 414 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGCGCTCAACTTCAGGT |
| Suggested primer 2: | CACACAAACACACCGGCTAC |