Insertion junction: LMJ.RY0402.213539_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.213539
Chromosome:chromosome 9
Location:4202198
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394213 DLT4,Tctex2b Dynein Light Chain; (1 of 4) PF03645 - Tctex-1 family (Tctex-1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCGACCGATGCGCCGTCACCATAATCC
Internal bar code:GTCGCCGTATAAAATCCATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:99.7674
LEAP-Seq n confirming:3003
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGACTGAGAGGCGGGTA
Suggested primer 2:ACACAGACAACTACGCGCAC