| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213555 |
| Chromosome: | chromosome 6 |
| Location: | 3131907 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCTTGACACGTCAGTCACGTCATTCTGA |
| Internal bar code: | CCGTCTGAAACCGAAATCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 84.4717 |
| LEAP-Seq n confirming: | 29163 |
| LEAP-Seq n nonconfirming: | 5361 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTCACTAACCGCTGTC |
| Suggested primer 2: | TGTGCAGCGTTGTAAAAAGG |