Insertion junction: LMJ.RY0402.213579_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213579
Chromosome:chromosome 3
Location:5206717
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g182650 PGA1 Phospholipid/glycerol acyltransferase; (1 of 2) K13511 - monolysocardiolipin acyltransferase (TAZ) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTCGCTCAACCGTTCTCGTGCTCAAAT
Internal bar code:GCGCGGGCGAGAGTGAGGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:99.9449
LEAP-Seq n confirming:1813
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGATTTTACAGGCGGAA
Suggested primer 2:TGATAGAAACCAGCTGTGCG