| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213607 |
| Chromosome: | chromosome 14 |
| Location: | 356715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTACCTTACCATGCCGTTCATGTTTGGC |
| Internal bar code: | GGACGAGACGTAACGACGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.0725 |
| LEAP-Seq n confirming: | 5341 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCGGTAGCGTCAATTCG |
| Suggested primer 2: | GCTCTGCCCCTACAACTCAG |