| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213622 |
| Chromosome: | chromosome 6 |
| Location: | 1544537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260050 | (1 of 27) IPR015947 - PUA-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCCTACGGCCGCATGCGGCGCCGGGGG |
| Internal bar code: | TCTGGGAGAGCCCTGGGCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 99.5974 |
| LEAP-Seq n confirming: | 1237 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCACCTCCATCAGAACGC |
| Suggested primer 2: | CACATTGGACTGACTGGTGG |