Insertion junction: LMJ.RY0402.213625_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.213625
Chromosome:chromosome 16
Location:1596744
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g653900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGATGAGCTGACCTCGTTCACGCCGTC
Internal bar code:AGTAACGACTCCGCTTCTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:99.0909
LEAP-Seq n confirming:218
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGATTCCAACCAGTCCAACA
Suggested primer 2:AAGCATTTCCTTGGCTGCTA