| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213628 |
| Chromosome: | chromosome 16 |
| Location: | 1997362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656750 | (1 of 2) PTHR12210//PTHR12210:SF70 - NUCLEAR LIM INTERACTOR-INTERACTING FACTOR-RELATED // AT02326P-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTACGTGAGGGGGTGTGCAAGAAGCGT |
| Internal bar code: | CGTCGGGCGGTGACTCTGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 99.7682 |
| LEAP-Seq n confirming: | 5164 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGCACCTCCTCTCACT |
| Suggested primer 2: | AGTCTGGTTATGTGCTGGGG |