| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213654 |
| Chromosome: | chromosome 6 |
| Location: | 2776580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271050 | URN1 | (1 of 1) 3.2.2.3 - Uridine nucleosidase / Uridine ribohydrolase; Inosine/uridine-preferring nucleoside hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGATATAGTGCAGGAGCAAGGCCGCGAG |
| Internal bar code: | TTAGAGCTCGATCTCTCGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 99.5242 |
| LEAP-Seq n confirming: | 4811 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGCCGTTACCTCGCTA |
| Suggested primer 2: | ATCTACCTGCACGACCCAAC |