| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213660 |
| Chromosome: | scaffold_18 |
| Location: | 265180 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre18.g750047 | FMG1-A | Flagella membrane glycoprotein, minor form | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCTCCCAGGGCGCTGGCTACTACCTGAT |
| Internal bar code: | CAGACGGTACGGACCTCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 112 |
| LEAP-Seq percent confirming: | 86.4407 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGACTCTACCAAGACCG |
| Suggested primer 2: | CTGAACACAAACACGGCATC |