Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.213674 |
Chromosome: | chromosome 2 |
Location: | 622727 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g077925 | (1 of 1) PTHR15628//PTHR15628:SF1 - FAMILY NOT NAMED // RWD DOMAIN-CONTAINING PROTEIN 3 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGGGAGGGATGCACGTGGGTCCGCTG |
Internal bar code: | GGAGGCAACACGAGACGAGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 683 |
LEAP-Seq percent confirming: | 59.8894 |
LEAP-Seq n confirming: | 2274 |
LEAP-Seq n nonconfirming: | 1523 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCACAGAGGCACAACACTA |
Suggested primer 2: | ACACGCACACACACACACAC |