| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213687 |
| Chromosome: | chromosome 4 |
| Location: | 2579944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222550 | (1 of 43) IPR011989 - Armadillo-like helical | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAAACAACGAAAACGAGGTCTGGTGCT |
| Internal bar code: | TCCGGGTCGGCGGAGACGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.7597 |
| LEAP-Seq n confirming: | 8304 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGGCCATACCTGTGT |
| Suggested primer 2: | AACTCTTCAATCTGCCGCAT |