| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213696 |
| Chromosome: | chromosome 13 |
| Location: | 4195606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592300 | (1 of 1) 1.3.1.91 - tRNA-dihydrouridine(20) synthase (NAD(P)(+)) / tRNA-dihydrouridine synthase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGTGCCCCAAAACGGCCCGAGACCCA |
| Internal bar code: | GATTGGCAGCATCGCGCGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.1851 |
| LEAP-Seq n confirming: | 4625 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAAACAACAGCGGTAGA |
| Suggested primer 2: | CGCATTCTCGTTTGTCTCAA |