Insertion junction: LMJ.RY0402.213699_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.213699
Chromosome:chromosome 5
Location:2944430
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCCCCGGGCTCTGGGGGTCATGAGTTA
Internal bar code:ACACCCGCGTTACCCTGCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:445
LEAP-Seq percent confirming:99.2409
LEAP-Seq n confirming:1961
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCTACTTGAGCTTGGTT
Suggested primer 2:CACGTGGGGAGGTAGGAGTA