Insertion junction: LMJ.RY0402.213699_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.213699
Chromosome:chromosome 5
Location:2944440
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGATGATGTGTACTATAATTGTACCCG
Internal bar code:GTGCATTTTGTATCGTAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:326
LEAP-Seq percent confirming:25.9649
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:211
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCTACTTGAGCTTGGTT
Suggested primer 2:CACGTGGGGAGGTAGGAGTA