| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213712 |
| Chromosome: | chromosome 9 |
| Location: | 1949030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395000 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATCACCAGGGAGGGGCGGGGGTCAAGG |
| Internal bar code: | TGCTCCGGCTACTTGTTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 74.8455 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTGTTGAGGGTTGCAG |
| Suggested primer 2: | CGTGAAGACCAAACGTGCTA |