| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213726 |
| Chromosome: | chromosome 2 |
| Location: | 4906043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103500 | (1 of 1) 1.14.13.169 - Transferred entry: 1.14.18.5 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATATTTGAACATCCAGTTGAACATCACAG |
| Internal bar code: | AGCGACACCGGAATCTGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.5759 |
| LEAP-Seq n confirming: | 3287 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAGTAGGGCGTGGCATA |
| Suggested primer 2: | TACTGCCACGTGTCCATGAT |