Insertion junction: LMJ.RY0402.213726_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213726
Chromosome:chromosome 16
Location:5047603
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCCAAGCGGGTCCCACTCGCGCACGGC
Internal bar code:TCTGCAGTACTGTCCGCAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:47.0588
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCGGTCAAAACGTGTTCC
Suggested primer 2:ACTGTGAGTCCCCACAAAGG