| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213736 |
| Chromosome: | chromosome 5 |
| Location: | 1074880 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGGTCGCCAAGCGACCGCCACACCATA |
| Internal bar code: | TTATCATAGCGCGCCTTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.5046 |
| LEAP-Seq n confirming: | 18478 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTCCCTGTCACAACGAT |
| Suggested primer 2: | GTTCCCATCCAAGCACTGTT |