| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213740 |
| Chromosome: | chromosome 12 |
| Location: | 1513247 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487150 | PGM20 | (1 of 2) PTHR23029//PTHR23029:SF24 - PHOSPHOGLYCERATE MUTASE // SUBFAMILY NOT NAMED; Putative phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTTGCTAGTCGCTACTGCCCTTGCATT |
| Internal bar code: | GCCGTCGCGCCTCGTGGGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.6563 |
| LEAP-Seq n confirming: | 4639 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAGTGAGTGCATTTGCC |
| Suggested primer 2: | ATGTGGTCACAACTGCGTGT |