| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213741 |
| Chromosome: | chromosome 3 |
| Location: | 4627797 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177250 | HAP3 | (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase; Haloperoxidase-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAAACAGCATGTGGACGGAAGTTATGAG |
| Internal bar code: | AGCCCAAACGGTCGTACCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 97.3745 |
| LEAP-Seq n confirming: | 1261 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCGCTCACAGAATGCAC |
| Suggested primer 2: | TCGATTGTCATAAGTGGGCA |