| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213743 |
| Chromosome: | chromosome 1 |
| Location: | 2050825 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010950 | (1 of 2) PF00023//PF12796//PF13920 - Ankyrin repeat (Ank) // Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCACCCCGTCCCACCCTCCGAATGC |
| Internal bar code: | GCATCGTCGCAGGCTAAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 98.6667 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCATGAGGACTGAGCAT |
| Suggested primer 2: | AAAAAGGGCACACACGAAAC |