| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213746 |
| Chromosome: | chromosome_12 |
| Location: | 1076401 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g490850 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACAACAGAGCACAGGGCGGCAGCAGTGTG |
| Internal bar code: | ATGCACTGCGTGGAGGTGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 81.2074 |
| LEAP-Seq n confirming: | 834 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAACTCTCCCCGTCACC |
| Suggested primer 2: | ATGAGGAATTCACCCCAACA |