| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213754 |
| Chromosome: | chromosome 7 |
| Location: | 6337985 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357250 | protein of unknown function; (1 of 1) PTHR24188//PTHR24188:SF41 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACTCAGAGGGATGTAGAGATCTGGGCG |
| Internal bar code: | ACCATCCACGACGCCCCCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.7275 |
| LEAP-Seq n confirming: | 2562 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCACGGACAGGTGTTTCT |
| Suggested primer 2: | AGCTCTTGCACCTGACCTGT |